Most read article(s)
MMP9 modulates the metastatic cascade and immune landscape for breast cancer anti-metastatic therapyInhibition of active MMP9 early during tumorigenesis suppresses tumor cell migration, invasion, and colony formation and tilts the balance towards anti-tumor immunity by activating CD8+ T cells.
Chemical genetic identification of GAK substrates reveals its role in regulating Na+/K+-ATPaseNovel GAK phosphorylation targets are identified using chemical genetic methods. One of the substrates is the α subunit of the Na+/K+-ATPase, phosphorylation of which is necessary for its surface trafficking from endosomes. Conserved functions of NAK family kinases are described.
TRAIL-induced variation of cell signaling states provides nonheritable resistance to apoptosisThis work summarizes cellular apoptotic and signaling response to TRAIL across 10 cell lines and for the first time links signaling diversity to nongenetic resistance. This high-dimensional, single-cell approach toward TRAIL resistance sets a standard in studying nongenetic resistance.
In vitro education of human natural killer cells by KIR3DL1Using NK cells isolated from individuals who lack the Bw4 epitope on HLA-B, Pugh et al reveal that KIR3DL1+ NK cells can be educated in vitro by co-culturing them with target cells that display the missing epitope.
Multiple membrane extrusion sites drive megakaryocyte migration into bone marrow blood vesselsMegakaryocytes enter bone marrow sinusoids to generate platelets by extrusion of internal membrane into the plasma membrane at fusion sites rather than microtubule-driven proplatelet extension.
Silica-based solid-phase extraction of cross-linked nucleic acid–bound proteinsThe 2C method allows the rapid and straightforward isolation of nucleic acid–protein complexes, greatly simplifying downstream applications for the study of DNA– and RNA–protein interactions.
![Distinct signals and immune cells drive liver pathology and glomerulonephritis in ABIN1[D485N] mice](https://www.life-science-alliance.org/sites/default/files/styles/lsa_thumbnail_image/public/externals/1826ba9c9b29cdb17704ada32e87ee01.jpg?itok=uRNfFQLZ)
Distinct signals and immune cells drive liver pathology and glomerulonephritis in ABIN1[D485N] miceThis article shows that liver and kidney damage in a lupus-prone mouse line occurs by different mechanisms and that only drugs targeting core components of signaling pathway, such as IRAK4, are able to suppress all facets of the disease.
The crystal structure of Staufen1 in complex with a physiological RNA sheds light on substrate selectivityCombination of in vitro and in vivo data show that RNA sequence influences Staufen target recognition and that protein–RNA base contacts are required for Staufen function in Drosophila.
Loss of miR-210 leads to progressive retinal degeneration in Drosophila melanogasterDepletion of miRNA-210 disrupts photoreceptor integrity and visual function in Drosophila melanogaster.
Dimerization and auto-processing induce caspase-11 protease activation within the non-canonical inflammasomeThis study provides a detailed molecular mechanism for caspase-11 activation within the non-canonical inflammasome, giving new insight into host defence against cytosolic bacterial infection.