Most read article(s)
Convergent evolution and horizontal gene transfer in Arctic Ocean microalgaeThe Arctic Ocean stands apart from other oceans. Using multiple genomes and transcriptomes from cultured and environmental sources, we found distinct genetic modifications in Arctic microalgae consistent with biogeographically constrained trait selectivity as an adaptive force in algal evolution.
Loss of NDR1/2 kinases impairs endomembrane trafficking and autophagy leading to neurodegenerationNDR1/2 kinases are essential for efficient endocytosis and protein clearance by autophagy. Neuron-specific dual deletion of NDR1 and NDR2 causes cortical and hippocampal neurodegeneration, implicating these kinases in neuronal protein homeostasis and health.
Concordance of MERFISH spatial transcriptomics with bulk and single-cell RNA sequencingSingle-cell RNA-seq is an important technology for capturing gene expression in individual cells. It has limitations as spatial context and accurate representation of cell types in the tissue are lost. Here, we show that MERFISH is comparable to scRNA-seq.
The mechanosensitive ion channel PIEZO1 promotes satellite cell function in muscle regenerationPIEZO1, a mechanosensitive ion channel, regulates Rho-GTPase activity and specifically accumulates at the midbody of muscle satellite cells to precisely control cell division for muscle regeneration.
Drosophila pVALIUM10 TRiP RNAi lines cause undesired silencing of Gateway-based transgenesDrosophila pVALIUM10 TRiP RNAi lines cause undesirable knockdown of transgenic reporters and overexpression lines generated with the help of the Gateway cloning technology by targeting attB sites.
Disrupted in renal carcinoma 2 (DIRC2/SLC49A4) is an H+-driven lysosomal pyridoxine exporterDIRC2 is an H+-driven lysosomal pyridoxine exporter involved in the regulation of cellular pyridoxine storage.
DSCR1 deficiency ameliorates the Aβ pathology of Alzheimer’s disease by enhancing microglial activityThe authors suggested that an important role of Down syndrome critical region 1 (DSCR1) in microglial activation is to provide a potential therapeutic target for microglial Aβ clearance in Alzheimer’s disease.
Revisiting degron motifs in human AURKA required for its targeting by APC/CFZR1AURKA degron motifs are redefined to show that the so-called N-terminal “A-box” is in fact a D-box, and the so-called “D-box” in the C-terminus is not a degron but a motif critical for the active, degradable conformation of AURKA.
Transcriptome analyses in infertile men reveal germ cell–specific expression and splicing patternsWe uncover extensive germ cell type–dependent transcriptome changes in human spermatogenesis and demonstrate the potential of alternative splicing in the regulation of spermatogenesis.
Laminin is the ECM niche for trophoblast stem cellsLaminin functions as an ECM niche factor for trophoblast stem cells and secures trophoblast stem cell expansion through its interactions with integrin.