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Resolution of the curse of dimensionality in single-cell RNA sequencing data analysisThis work formulates a noise reduction method, RECODE, which resolves the curse of dimensionality in noisy high-dimensional data, including scRNA-seq data, for effective downstream analyses.
Islet Gene View—a tool to facilitate islet researchIslet Gene View (IGW) is a web resource that makes information on gene expression in human islets from donors easily accessible to the scientific community. The relationship of global RNA expression from 188 donor-islets with islet phenotypes is explored.
RNA-sequencing of single cholangiocyte-derived organoids reveals high organoid-to organoid variabilityWhereas batch variation between organoid cultures is low, inter-culture heterogeneity can be large, even within one well, which might stem from microenvironmental differences within the culture.
ERK2 MAP kinase regulates SUFU binding by multisite phosphorylation of GLI1As a mechanism of crosstalk, potentially relevant to cancer and developmental signaling, ERK2 MAP kinase phosphorylates the Hedgehog-pathway transcription factor GLI1 on three sites, promoting release of the negative regulator SUFU and the consequent activation of GLI1.
Novel DNM1L variants impair mitochondrial dynamics through divergent mechanismsNovel DNM1L variants underlie a spectrum of clinical phenotypes and impair mitochondrial and peroxisomal dynamics via divergent mechanisms, with effects on DRP1 protein stability, GTPase activity, and oligomerisation in vitro.
Measuring cystic fibrosis drug responses in organoids derived from 2D differentiated nasal epitheliaThis work describes an optimized assay to determine CFTR drug responses in people with cystic fibrosis, using nasal-airway organoids that are generated from 2D differentiated epithelial monolayers.
Chr21 protein–protein interactions: enrichment in proteins involved in intellectual disability, autism, and late-onset Alzheimer’s diseaseA large-scale (82) yeast two-hybrid screen focusing on chromosome 21 proteins and their direct interactors revealed enrichment in proteins linked to synapse plasticity, autism, and Alzheimer’s disease.
Human milk inhibits some enveloped virus infections, including SARS-CoV-2, in an intestinal modelHuman milk blocks some enveloped, but not non-enveloped, virus infections in a primary human fetal intestinal model and the transcriptomic data points towards inhibition of entry mediated by ATP1A1.
Comparative functional genomics identifies unique molecular features of EPSCsThe authors provide a comprehensive resource on proteomics, transcriptomic, and epigenetic level details of EPSCs to shed light on possible molecular pathways regulating their expanded pluripotency potential.
Bivalent binding of p14ARF to MDM2 RING and acidic domains inhibits E3 ligase functionThis study shows that MDM2 acidic and zinc finger regions weakly interact with the RING domain and a fragment of p14ARF strengthens this interaction to inhibit MDM2 ubiquitin ligase activity.