Most read article(s)
Evaluation of colorectal cancer subtypes and cell lines using deep learningA deep learning approach refines the state-of-the-art subtypes of colorectal cancer and evaluates the fidelity of cell lines that model cancer.
Cx3cr1-deficient microglia exhibit a premature aging transcriptomeGyoneva et al use RNA-seq to show that Cx3cr1-deficient microglia in young mice display a gene expression profile similar to microglia in aged mice, suggesting premature microglial aging.
Chemical genetic identification of GAK substrates reveals its role in regulating Na+/K+-ATPaseNovel GAK phosphorylation targets are identified using chemical genetic methods. One of the substrates is the α subunit of the Na+/K+-ATPase, phosphorylation of which is necessary for its surface trafficking from endosomes. Conserved functions of NAK family kinases are described.
MERLIN: a novel BRET-based proximity biosensor for studying mitochondria–ER contact sitesMERLIN is a novel biosensor that generates a BRET signal with a signal-to-noise ratio that is sufficient to enable sensing the proximity between the mitochondria and the ER without forcing interaction or establishing artificial connections at the MERCs.
Expression of AXL receptor tyrosine kinase relates to monocyte dysfunction and severity of cirrhosisImmune dysfunction determines morbidity and mortality in liver cirrhosis. Distinct AXL-expressing circulating monocytes, which regulate antimicrobial responses, expand with progression of the disease.
MMP9 modulates the metastatic cascade and immune landscape for breast cancer anti-metastatic therapyInhibition of active MMP9 early during tumorigenesis suppresses tumor cell migration, invasion, and colony formation and tilts the balance towards anti-tumor immunity by activating CD8+ T cells.
TspanC8 tetraspanins differentially regulate ADAM10 endocytosis and half-lifeThis study demonstrates that the tetraspanins Tspan5 and Tspan15 which directly associate with the metalloprotease ADAM10 have an opposite impact on its endocytosis and half-life.
ADAP1 promotes invasive squamous cell carcinoma progression and predicts patient survivalADAP1, a GTPase-activating protein (GAP) for the small GTPase ARF6, is a strong predictor of poor survival in early-stage squamous cell carcinoma patients and a critical mediator of TGF-β-induced invasive cell migration by facilitating basement membrane breakdown.
Essential functions of Runx/Cbfβ in gut conventional dendritic cells for priming Rorγt+ T cellsLoss of Runx/Cbfβ complexes during DC development leads to a loss of gut CD103+CD11b+ cDC2s, which was accompanied with impaired differentiation of both Rorγt+ Th17 cells and type 3 Rorγt+ Treg cells.
Phenotypic proteomic profiling identifies a landscape of targets for circadian clock–modulating compoundsThis study provides comprehensive insights into the mechanism of action and cellular effects of circadian period–modulating compounds, which is critical for clearly defining molecular targets to modulate daily rhythms for therapeutic benefit.