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Autoimmune anti-DNA and anti-phosphatidylserine antibodies predict development of severe COVID-19COVID-19 induces high levels of autoimmune anti-DNA and anti-phosphatidylserine antibodies that are detected in some patients upon hospital admission and predict later development of severe disease.
Laminin is the ECM niche for trophoblast stem cellsLaminin functions as an ECM niche factor for trophoblast stem cells and secures trophoblast stem cell expansion through its interactions with integrin.
High levels of TFAM repress mammalian mitochondrial DNA transcription in vivoThe TFAM-to-mtDNA ratio is critically important and must be maintained within a physiological range to allow normal mtDNA expression and prevent nucleoid hypercompaction in different mouse tissues.
TMPRSS2 and furin are both essential for proteolytic activation of SARS-CoV-2 in human airway cellsThe spike protein S of SARS-CoV-2 is activated by TMPRSS2 and furin. Inhibition of either one of these proteases can render the S protein unable to mediate virus entry and, therefore, provides a promising approach for COVID-19 treatment.
Evaluating CRISPR-based prime editing for cancer modeling and CFTR repair in organoidsHere, the authors evaluate prime editing in human adult stem cell–derived organoids. TP53 mutations are modeled in hepatocyte and colon organoids and the clinical application of prime editing is evaluated by repairing mutations in CFTR without genome-wide off-targets.
Tumor-intrinsic response to IFNγ shapes the tumor microenvironment and anti–PD-1 response in NSCLCUsing an immunocompetent mouse model of NSCLC, this study demonstrates that tumor-intrinsic response to IFNγ determines response to anti–PD-1 through alterations in the tumor microenvironment.
Paralogous synthetic lethality underlies genetic dependencies of the cancer-mutated gene STAG2The context-dependency of genetic interactions of the cohesin gene STAG2, specifically STAG1 and the iron regulatory gene IREB2, are revealed by CRISPR-Cas9 screening in three different cellular backgrounds.
An extended DNA-free intranuclear compartment organizes centrosome microtubules in malaria parasitesMalaria arises during the proliferation of Plasmodium spp. in human blood, whereas the underlying atypical cell division mechanisms remain poorly understood. This study uses advanced imaging to dissect dynamics and organization of the centrosome, a key division regulator.
Biallelic ADPRHL2 mutations in complex neuropathy affect ADP ribosylation and DNA damage responseThis work studies known and new ADPRHL2 mutations with different disease mechanisms overall indicating that loss of nuclear ARH3 alone is pathogenic via dysregulated nuclear ADP ribosylation.
Exosome-mediated delivery of CRISPR/Cas9 for targeting of oncogenic KrasG12D in pancreatic cancerThis work identifies the use of exosomes to specifically deliver CRISPR/Cas9 to target oncogenic KrasG12D mutation in pancreatic cancer as a nonviral therapeutic strategy.