Stem Cells
- Origin and segregation of the human germline
Human primordial germ cells originate in peri-gastrulation embryos from the posterior epiblast precursors with an obligatory role for TFAP2A, which is redundant for contribution to the amnion.
- Evaluation of genetic demultiplexing of single-cell sequencing data from model species
The authors demonstrate that scRNA-seq sample pooling followed by genetics-based separation of individuals is an effective means to identify individual samples in a variety of commonly studied species.
- Validating organoid-derived human intestinal monolayers for personalized therapy in cystic fibrosis
Ussing chamber studies of intestinal organoid monolayers compare favorably to established preclinical tools to assess individual responses to CFTR modulator drugs in people with cystic fibrosis.
- SMN promotes mitochondrial metabolic maturation during myogenesis by regulating the MYOD-miRNA axis
SMN contributes to muscle maturation by binding to the promoter regions of MYOD1 and miRNAs and regulating the expression of these molecules during myogenesis.
- Trans-differentiation of trophoblast stem cells: implications in placental biology
Formation of trophendothelial cells is associated with trophoblast differentiation and is regulated by HES1. Trophoblast differentiation–induced TRAIL secretion directs preferential demise of ECs located in their vicinity, aiding trophoblast vasculogenic mimicry.
- The mechanosensitive ion channel PIEZO1 promotes satellite cell function in muscle regeneration
PIEZO1, a mechanosensitive ion channel, regulates Rho-GTPase activity and specifically accumulates at the midbody of muscle satellite cells to precisely control cell division for muscle regeneration.
- Cross talk between glucose metabolism and immunosuppression in IFN-γ–primed mesenchymal stem cells
This study reveals a novel relationship between mesenchymal stem cell immunomodulation and metabolism and provides a new strategy to improve their therapeutic efficacy in inflammatory diseases.
- Meiotic DNA breaks activate a streamlined phospho-signaling response that largely avoids protein-level changes
The meiotic DNA break response is shown to involve a large number of phosphorylation changes and a clearly detectable transcriptional response. By contrast, the meiotic proteome remains remarkably unchanged in response to breaks.
- Comparative functional genomics identifies unique molecular features of EPSCs
The authors provide a comprehensive resource on proteomics, transcriptomic, and epigenetic level details of EPSCs to shed light on possible molecular pathways regulating their expanded pluripotency potential.
- Activity-based probe profiling of RNF12 E3 ubiquitin ligase function in Tonne-Kalscheuer syndrome
The authors use photocrosslinking activity-based probes to monitor RNF12/RLIM E3 ubiquitin ligase activity and report the impact of Tonne-Kalscheuer syndrome (TOKAS) patient variants on catalysis.