Molecular Biology
- Cell-free chromatin immunoprecipitation to detect molecular pathways in heart transplantation
Cell-free DNA chromatin immunoprecipitation detects molecular pathways and profiles the sources of tissue injury in heart transplant patients.
- A targeted sequencing extension for transcript genotyping in single-cell transcriptomics
We describe the targeted sequencing extension and provide two proof-of-concept examples of how it can assist with understanding the impact of genetic variants.
- Huntingtin loss in hepatocytes is associated with altered metabolism, adhesion, and liver zonation
Lifelong huntingtin loss in mouse hepatocytes is associated with altered metabolism, adhesion, and liver zonation which mimics the phenotypes caused by impaired B-catenin function.
- Comprehensive analysis of the lysine succinylome in fish oil-treated prostate cancer cells
Through a global lysine succinylation analysis of prostate cancer cells treated with fish oil, a significant number of lysine succinylation sites have been identified and FO-dependent GOT2 succinylation status may have a potential to inhibit building block generation.
- The arginine methyltransferase PRMT5 promotes mucosal defense in the intestine
Arginine methyltransferase PRMT5 promotes select colonic goblet cell-associated gene programs and facilitates host defense against enteric pathogens.
- Tumor necrosis factor mediates USE1-independent FAT10ylation under inflammatory conditions
The finding of TNF-dependent FAT10ylation in the absence of USE1 identifies seven additional, so far unknown FAT10-reactive E2 conjugating enzymes.
- Allotopic expression of COX6 elucidates Atco-driven co-assembly of cytochrome oxidase and ATP synthase
Relocation of COX6 from nuclear to the mitochondrial genome helps unveil the role of Atco (Atp9–Cox6 complex) in coupling the biogenesis of cytochrome oxidase and ATP synthase in yeast mitochondria.
- A novel antifolate suppresses growth of FPGS-deficient cells and overcomes methotrexate resistance
Mechanistic characterization of a novel, lipophilic antifolate that suppresses growth of FPGS-deficient cells that are linked to methotrexate resistance and relapse in cancer patients.
- SF3B1 mutation–mediated sensitization to H3B-8800 splicing inhibitor in chronic lymphocytic leukemia
Understanding the pathogenic mechanisms of SF3B1 mutation can help unravel their contribution in patients’ worse prognosis. The cytotoxic effects and delayed leukemic infiltration induced by H3B-8800 support the potential use of SF3B1 inhibitors as a novel therapy in CLL.
- ATP-independent substrate recruitment to proteasomal degradation in mycobacteria
Mycobacterial proteasomes ensure survival during stress by recruiting substrates for degradation via ring-shaped activators. This study elucidates the mechanism of ATP-independent substrate recruitment through the bacterial proteasome activator (Bpa), revealing the determinants for substrate recognition and efficient degradation.