Molecular Biology
- Variety in the USP deubiquitinase catalytic mechanism
Quantitative analysis shows that USP family members have varying catalytic triads. Their third and fourth catalytic residues change in role and importance for nucleophilic attack and catalysis.
- Correction: Profiling subcellular localization of nuclear-encoded mitochondrial gene products in zebrafish
The vast majority (99%) of mitochondrial proteins are encoded by the nuclear genome, synthesized in the cytosol and imported into the organelle. Here we study the principles of mitochondrial import in vivo from the RNA perspective using zebrafish.
- Knockout of PA200 improves proteasomal degradation and myelination in a proteotoxic neuropathy
Proteasome Activator 200 is upregulated in a neuropathy model in which protein degradation by the proteasome is impaired. Knocking out PA200 unexpectedly rescued proteostasis and prevented neuropathy.
- Zebrafish tsc1 and cxcl12a increase susceptibility to mycobacterial infection
Knockdown of miR-126 increases susceptibility to mycobacterial infection which can be independently reversed by targeting Tsc1/mTOR or ccr2 implicating macrophage function.
- FERM domain–containing proteins are active components of the cell nucleus
The information we gathered from the literature reveals that FERM domain proteins perform important and diverse functions not only in the cytoplasm, but also in the cell nucleus.
- Integrated analysis of RNA-seq datasets reveals novel targets and regulators of COVID-19 severity
This work observed differential expression of genes related to the endomembrane system, specific granules, T cell receptor complex, and SPI1 and TP53 target genes exclusively in severe COVID-19 patients.
- Synaptobrevin2 monomers and dimers differentially engage to regulate the functional trans-SNARE assembly
This work demonstrates different factors controlling the synaptobrevin2 dimer-to-monomer ratio, which has ramifications in fusion pore stability.
- USP27X variants underlying X-linked intellectual disability disrupt protein function via distinct mechanisms
This study uncovers mechanisms by which X-linked intellectual disability disorder 105 (#300984; OMIM) variants alter USP27X biology and function.
- Tmprss2 maintains epithelial barrier integrity and transepithelial sodium transport
The membrane-bound serine protease Tmprss2 is required for ENaC-mediated transepithelial sodium and EpCAM-mediated paracellular transport in kidney cells.
- Gene architecture is a determinant of the transcriptional response to bulky DNA damages
A comparative genomic study of the transcriptomic response to bulky DNA damages uncovers novel response genes and the features of their structure that allow them to be better repaired and transcribed.