User profiles for N. N. Giakoumakis

Nikolaos Nikiforos Giakoumakis

Research Officer IRB Barcelona
Verified email at irbbarcelona.org
Cited by 552

easyFRAP: an interactive, easy-to-use tool for qualitative and quantitative analysis of FRAP data

…, P Kotsantis, IE Symeonidou, NN Giakoumakis… - …, 2012 - academic.oup.com
We present easyFRAP, a versatile tool that assists quantitative and qualitative analysis of
fluorescence recovery after photobleaching (FRAP) data. The user can handle simultaneously …

[HTML][HTML] Disruption of mitochondrial dynamics triggers muscle inflammation through interorganellar contacts and mitochondrial DNA mislocation

…, E Frank, NN Giakoumakis… - Nature …, 2023 - nature.com
Some forms of mitochondrial dysfunction induce sterile inflammation through mitochondrial
DNA recognition by intracellular DNA sensors. However, the involvement of mitochondrial …

EasyFRAP-web: a web-based tool for the analysis of fluorescence recovery after photobleaching data

…, MA Rapsomaniki, NN Giakoumakis… - Nucleic acids …, 2018 - academic.oup.com
Understanding protein dynamics is crucial in order to elucidate protein function and interactions.
Advances in modern microscopy facilitate the exploration of the mobility of fluorescently …

The licensing factor Cdt1 links cell cycle progression to the DNA damage response

A Kanellou, NN Giakoumakis… - Anticancer …, 2020 - ar.iiarjournals.org
The maintenance of genome integrity is essential for cellular survival and propagation. It
relies upon the accurate and timely replication of the genetic material, as well as the rapid …

[HTML][HTML] Mutational signatures reveal the role of RAD52 in p53-independent p21-driven genomic instability

…, A Polyzos, A Kotsinas, I Svolaki, NN Giakoumakis… - Genome biology, 2018 - Springer
Background Genomic instability promotes evolution and heterogeneity of tumors. Unraveling
its mechanistic basis is essential for the design of appropriate therapeutic strategies. In a …

[HTML][HTML] CK1δ restrains lipin-1 induction, lipid droplet formation and cell proliferation under hypoxia by reducing HIF-1α/ARNT complex formation

M Kourti, G Ikonomou, NN Giakoumakis… - Cellular signalling, 2015 - Elsevier
Proliferation of cells under hypoxia is facilitated by metabolic adaptation, mediated by the
transcriptional activator Hypoxia Inducible Factor-1 (HIF-1). HIF-1α, the inducible subunit of HIF…

[PDF][PDF] Proteostasis failure and mitochondrial dysfunction leads to aneuploidy-induced senescence

…, C Santos-Tapia, D Romão, NN Giakoumakis… - Developmental cell, 2021 - cell.com
Aneuploidy, an unbalanced number of chromosomes, is highly deleterious at the cellular
level and leads to senescence, a stress-induced response characterized by permanent cell-…

Analysis of protein kinetics using fluorescence recovery after photobleaching (FRAP)

NN Giakoumakis, MA Rapsomaniki… - Light Microscopy: Methods …, 2017 - Springer
Fluorescence recovery after photobleaching (FRAP) is a cutting-edge live-cell functional
imaging technique that enables the exploration of protein dynamics in individual cells and thus …

Direct binding of Cdt2 to PCNA is important for targeting the CRL4Cdt2 E3 ligase activity to Cdt1

A Hayashi, NN Giakoumakis… - Life Science …, 2018 - life-science-alliance.org
The CRL4 Cdt2 ubiquitin ligase complex is an essential regulator of cell-cycle progression
and genome stability, ubiquitinating substrates such as p21, Set8, and Cdt1, via a display of …

[HTML][HTML] Expansion microscopy of the chick embryo neural tube to overcome molecular crowding at the centrosomes-cilia

A Wilmerding, P Espana-Bonilla, NN Giakoumakis… - STAR protocols, 2023 - Elsevier
We describe an optimized protocol for application of expansion microscopy (ExM) on chick
neural tube (NT) which enables different oriented nanoscale resolution imaging of the …