Lymph node stromal cells acquire peptide–MHCII complexes from dendritic cells and induce antigen-specific CD4+ T cell tolerance

…, FV Duraes, L Potin, F Capotosti, D Brighouse… - Journal of Experimental …, 2014 - rupress.org
… 3 d after irradiation, the number of hematopoietic cells in LN … 2 D). These results indicated
that LNSCs transiently acquire … In brief, LN cells were cultured for 5–7 d and washed daily to …

IFN-γ–dependent tumor-antigen cross-presentation by lymphatic endothelial cells promotes their killing by T cells and inhibits metastasis

L Garnier, R Pick, J Montorfani, M Sun, D Brighouse… - Science …, 2022 - science.org
Tumor-associated lymphatic vessels promote metastasis and regulate antitumor immune
responses. Here, we assessed the impact of cytotoxic T cells on the local lymphatic vasculature …

[HTML][HTML] IDO-orchestrated crosstalk between pDCs and Tregs inhibits autoimmunity

C Lippens, FV Duraes, J Dubrot, D Brighouse… - Journal of …, 2016 - Elsevier
… For qPCR experiments, pDCs, cDCs, B cells and macrophages were recovered from LN
after digestion with an enzymatic mix containing collagenase D (1 mg/mL) and DNAse I (10 μg/…

MHC Class II antigen presentation by lymphatic endothelial cells in tumors promotes intratumoral regulatory T cell–suppressive functions

…, J Dubrot, J Angelillo, G Harlé, D Brighouse… - Cancer immunology …, 2021 - AACR
D, FACS histograms showing MHCII expression levels by LECs in indicated organs at
days 11 and 15 (left), and quantitative histograms (right). Data are representative of two …

Macroautophagy in lymphatic endothelial cells inhibits T cell–mediated autoimmunity

G Harlé, C Kowalski, J Dubrot, D Brighouse… - Journal of Experimental …, 2021 - rupress.org
… Therefore, we characterized T reg cell frequencies and Foxp3 expression 20 d after CIA …
T reg cells (RFP + cells) were sorted from dLNs of BM chimeras 26 d after CIA induction and …

Ag-presenting CpG-activated pDCs prime Th17 cells that induce tumor regression

L Guéry, J Dubrot, C Lippens, D Brighouse, P Malinge… - Cancer research, 2014 - AACR
… A–D, results are representative of at least three independent experiments. E–G, WT and
pIII+IV−/− mice were injected in the flank (sc) with PBS or CpG-B, and pDCs from dLN were …

Absence of MHC-II expression by lymph node stromal cells results in autoimmunity

…, G Harlé, A Schlaeppi, D Brighouse… - Life science …, 2018 - life-science-alliance.org
… In (C) and (D), animals were treated with FTY720 every day for the last 6 d, and with IFN-γ 6
d … (B–D) Data are pooled from at least nine tissue sections from three individual mice/group. *…

Intratumoral CpG-B promotes antitumoral neutrophil, cDC, and T-cell cooperation without reprograming tolerogenic pDC

M Humbert, L Guery, D Brighouse, S Lemeille… - Cancer research, 2018 - AACR
… Cell isolation from tumors was performed as follows: tumors were digested chemically with
collagenase D (1 mg/mL; Roche) and DNAse I (10 mg/mL; Roche) and mechanically. Dead …

[HTML][HTML] pDC therapy induces recovery from EAE by recruiting endogenous pDC to sites of CNS inflammation

…, C Lippens, K Steinbach, J Dubrot, D Brighouse… - Journal of …, 2016 - Elsevier
… by digesting organ fragments with an enzymatic mix containing collagenase D (1 mg/mL)
and DNAse I (10 μg/mL) (Roche) in HBSS [14]. For liver and SC, single cell suspensions were …

Does electroconvulsive therapy (ECT) have any role in the management of intractable thalamic pain?

S McCance, K Hawton, D Brighouse, C Glynn - Pain, 1996 - Elsevier
Three patients with intractable thalamic pain unresponsive to a range of previous treatments
received a course of six bilateral electroconvulsive therapy (ECT) treatments over 2 weeks. …