… The initial oven temperature of 100 C was maintained for 1 min and then raised by 4 C/min
to 320 C with a further 4 min of hold time. The gas flow was set to obtain a constant linear …

… The assembled genome size was 4.2 Mb with a G + C content of 43.3% (Table 1). Of the
3544 genes, 1687 (47.6%) were hypothetical proteins without a putative function assigned. O. …

… LPMCs were isolated from colons upon incubation with 5 mM EDTA at 37 C for 30 min,
followed by further digestion with collagenase IV and DNase at 37 C for 1 h. Cells were then …

… Before implantation, eggs were kept at 28C and manually dechorionated at 1 day … C. This
temperature was chosen as an intermediate between 37C (optimal cell temperature) and 28C (…

… The addition of the c-Met inhibitor crizotinib to the water in which the larvae were kept
reduced the migration and proliferation of UM cells expressing c-Met. This indicates the …

… at 95 C for 3 min, followed by 25 cycles consisting of denaturation (95 C for 30 s), annealing
(55 C for 30 s) and extension (72 C for 30 s), and a final extension step at 72 C for 5 min. …

… C for 30 min, followed by further digestion with collagenase IV and DNase at 37C for 1 h.
Cells were then separated with a Percoll density gradient (Sigma-Aldrich, St. Louis, MO, USA). …

… In summary, 2.5 × 10 4 cells·well −1 were incubated with Calcein AM for 30 min at 37 C
and then washed with PBS. Then, iNKT cells were added to each well at 8 : 1 E : T ratio for 4h. …

… (C, D) Comparison of CD161 expression and absolute numbers among CD4 − tetramer +
(C) or tetramer-negative (D) cells isolated from HDs (HD, white bars), UC patients (blue bars), …

A case of accidental intracranial penetration of a 29-cm-long portion of nasogastric tube (NGT)
in a 27-year-old female victim of a severe homicidal skull and brain trauma is reported. …