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Status |
Public on Sep 05, 2018 |
Title |
CARM1 Methylates MED12 to Regulate its RNA Binding Ability |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
The coactivator-associated arginine methyltransferase (CARM1) functions as a regulator for transcription, splicing and chromatin regulation by methylating a diverse array of substrates. In this study, we used CARM1 substrate antibodies to perform immunoprecipitation coupled with mass spectrometry (IP-MS) in MEFs and identified Mediator Subunit 12 (MED12) as a novel substrate for CARM1. ChIP-seq analysis using CARM1, MED12 and H3R17me2a (represents ‘CARM1 activity’) antibodies revealed that MED12 targeted by CARM1 is recruited to the EREs (estrogen-responsive elements). Additionally, RT-PCR studies showed that the MED12R1899K mutation disrupting the methylation site reduced the expression of ER-target genes. Thus, MED12 methylation targets it to ER-specific enhancers and positively modulates transcription of Estrogen-driven genes.
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Overall design |
ChIP-seq of CARM1, MED12 and H3R17me2a in MCF7 cells
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Contributor(s) |
Cheng D, Lu Y, Vemulapalli V, Bedford M |
Citation(s) |
30456381 |
Submission date |
Sep 09, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Yue Lu |
Organization name |
MD Anderson Cancer Center
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Department |
Epigenetics and Molecular Carcinogenesis
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Street address |
1808 Park Road 1C
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City |
Smithville |
State/province |
TX |
ZIP/Postal code |
78957 |
Country |
USA |
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Platforms (1) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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Samples (4)
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Relations |
BioProject |
PRJNA295184 |
SRA |
SRP063514 |