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| Status |
Public on Sep 06, 2018 |
| Title |
Expression analysis of PC3 cells treated with scramble AON or AON directed against MBNL1 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We transfected PC3 cells with 100nM of a scrambled antisense oligonucleotide (AON) and an AON directed against MBNL1 exon 7 (36 basepairs) in order to skip the latter. Cells were harvested at 72h post-transfection and RNAseq was performed with ribozero depletion.
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| Overall design |
For RNA-Seq library preparation we followed the Illumina TruSeq RNA Sample Preparation Kit v2 manual. At least 70 million, 75bp long paired end reads were mapped to the GRCh37/hg19 version of the human genome per replicate using STAR 2.4.2a (doi: 10.1093/bioinformatics/bts635) using the default parameters.
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| Contributor(s) |
TABAGLIO T, WOLLMANN H, LOW DH, GOY P |
| Citation(s) |
30456384 |
| Submission date |
May 14, 2018 |
| Last update date |
Mar 27, 2019 |
| Contact name |
Diana HP Low |
| E-mail(s) |
dlow@imcb.a-star.edu.sg
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| Organization name |
Institute of Molecular and Cell Biology
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| Lab |
Chromatin, Epigenetics & Differentiation
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| Street address |
#03-06, 61 Biopolis Drive, Proteos
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| City |
Singapore |
| ZIP/Postal code |
138673 |
| Country |
Singapore |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA471253 |
| SRA |
SRP145589 |
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