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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 10, 2017 |
| Title |
Context-dependent effects of Acf1 assessed in high throughput |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
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| Summary |
We applied a multiplexed genomic tethering assay (Brueckner, Epigenetics Chromatin, 2016; PMID:27777628) to elucidate the function of ACF1, the large subunit of the ACF nucleosome sliding complex in drosophila Kc167 cells. Targeting a Gal4DBD-ACF1 fusion to several hundred integrated reporters revealed a context-dependent repressive effect which is modulated by gene activity and chromatin state.
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| Overall design |
TRIP assay in drosophila Kc167 cells combined with targeted recruitment of Gal4-Acf1 to integrated 5xGa4UAS-pMT-GFP reporters. Two technical replicate experiments of TRIP pool transfected with Gal4-Acf1, Gal4, Acf1 or Gal4-HP1 performed by day 2 after transient transfection.
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| Contributor(s) |
Brueckner L, Scacchetti A, Becker P, van Steensel B |
| Citation(s) |
30456345 |
| Submission date |
Nov 09, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Bas van Steensel |
| E-mail(s) |
b.v.steensel@nki.nl
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| Phone |
+ 31 20 512 2040
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| Fax |
+31 20 669 1383
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| URL |
http://www.nki.nl/nkidep/vansteensel
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| Organization name |
Netherlands Cancer Institute
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| Department |
division of Molecular Biology
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| Lab |
van Steensel group
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| Street address |
Plesmanlaan 121
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| City |
Amsterdam |
| ZIP/Postal code |
1066 CX |
| Country |
Netherlands |
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| Platforms (3) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
| GPL16479 |
Illumina MiSeq (Drosophila melanogaster) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
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| Samples (18)
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| GSM2849964 |
cDNA reads, Gal4, repl 2 |
| GSM2849965 |
cDNA reads, ACF1, repl 1 |
| GSM2849966 |
cDNA reads, ACF1, repl 2 |
| GSM2849967 |
cDNA reads, Gal4-HP1a, repl 1 |
| GSM2849968 |
cDNA reads, Gal4-HP1a, repl 2 |
| GSM2849969 |
gDNA reads, Gal4-ACF1, repl 1 |
| GSM2849970 |
gDNA reads, Gal4-ACF1, repl 2 |
| GSM2849971 |
gDNA reads, Gal4, repl 1 |
| GSM2849972 |
gDNA reads, Gal4, repl 2 |
| GSM2849973 |
gDNA reads, ACF1, repl 1 |
| GSM2849974 |
gDNA reads, ACF1, repl 2 |
| GSM2849975 |
gDNA reads, Gal4-HP1, repl 1 |
| GSM2849976 |
gDNA reads, Gal4-HP1, repl 2 |
| GSM2849977 |
Kc167_mapping reads |
| GSM2849978 |
Kc167_spike-in gDNA reads |
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| Relations |
| BioProject |
PRJNA417865 |
| SRA |
SRP124746 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE106759_RAW.tar |
10.0 Kb |
(http)(custom) |
TAR (of TXT) |
| GSE106759_TRIP_ACF1.csv.gz |
82.9 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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