A toolkit for high-throughput, cross-species gene engineering in Drosophila

Radoslaw K Ejsmont; Mihail Sarov; Sylke Winkler; Kamil A Lipinski; Pavel Tomancak

doi:10.1038/nmeth.1334

A toolkit for high-throughput, cross-species gene engineering in Drosophila

Nat Methods. 2009 Jun;6(6):435-7. doi: 10.1038/nmeth.1334.

Authors

Radoslaw K Ejsmont¹, Mihail Sarov, Sylke Winkler, Kamil A Lipinski, Pavel Tomancak

Affiliation

¹ Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.

PMID: 19465918
DOI: 10.1038/nmeth.1334

Abstract

We generated two complementary genomic fosmid libraries for Drosophila melanogaster and Drosophila pseudoobscura that permit seamless modification of large genomic clones by high-throughput recombineering and direct transgenesis. The fosmid transgenes recapitulated endogenous gene expression patterns. These libraries, in combination with recombineering technology, will be useful to rescue mutant phenotypes, allow imaging of gene products in living flies and enable systematic analysis and manipulation of gene activity across species.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Animals, Genetically Modified / genetics*
Chromosome Mapping / methods*
Cloning, Molecular / methods*
Drosophila / genetics*
Gene Library*
Genetic Engineering / methods*