A protocol for combined Photinus and Renilla luciferase quantification compatible with protein assays

Mathias Hampf; Manfred Gossen

doi:10.1016/j.ab.2006.04.046

A protocol for combined Photinus and Renilla luciferase quantification compatible with protein assays

Anal Biochem. 2006 Sep 1;356(1):94-9. doi: 10.1016/j.ab.2006.04.046. Epub 2006 May 12.

Authors

Mathias Hampf¹, Manfred Gossen

Affiliation

¹ Max Delbrück Center for Molecular Medicine, 13125 Berlin, Germany.

PMID: 16750160
DOI: 10.1016/j.ab.2006.04.046

Abstract

We established a quantitative reporter gene protocol, the P/Rluc assay system, allowing the sequential measurement of Photinus and Renilla luciferase activities from the same extract. Other than comparable commercial reporter assay systems and their noncommercial counterparts, the P/Rluc assay system was formulated under the aspect of full compatibility with standard methods for protein assays. This feature greatly expands the range of applications for assay systems quantifying the expression of multiple luciferase reporters.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Enzyme Inhibitors / pharmacology
Fireflies / enzymology
Fireflies / genetics
Genes, Reporter
HeLa Cells
Humans
Luciferases, Firefly / analysis*
Luciferases, Firefly / antagonists & inhibitors
Luciferases, Firefly / genetics
Luciferases, Renilla / analysis*
Luciferases, Renilla / genetics
Luminescent Measurements / methods
Renilla / enzymology
Renilla / genetics

Substances

Enzyme Inhibitors
luciferase, Photinus
Luciferases, Renilla
Luciferases, Firefly