Differentiation of mouse embryonic stem cells into a defined neuronal lineage

Nat Neurosci. 2004 Sep;7(9):1003-9. doi: 10.1038/nn1301.

Abstract

Although it has long been known that cultured embryonic stem cells can generate neurons, the lineage relationships with their immediate precursors remain unclear. We report here that selection of highly proliferative stem cells followed by treatment with retinoic acid generated essentially pure precursors that markers identified as Pax-6-positive radial glial cells. As they do in vivo, these cells went on to generate neurons with remarkably uniform biochemical and electrophysiological characteristics.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Bicuculline / pharmacology
  • Blotting, Western
  • Calmodulin-Binding Proteins / metabolism
  • Carrier Proteins / metabolism
  • Cell Count / methods
  • Cell Differentiation / drug effects
  • Cell Differentiation / physiology*
  • Cell Differentiation / radiation effects
  • Cell Lineage / physiology*
  • Cells, Cultured
  • Drug Interactions
  • Electric Stimulation / methods
  • Embryo, Mammalian / cytology*
  • Excitatory Amino Acid Agonists / pharmacology
  • Excitatory Amino Acid Antagonists / pharmacology
  • Eye Proteins
  • Fatty Acid-Binding Protein 7
  • Fatty Acid-Binding Proteins
  • GABA Antagonists / pharmacology
  • Glial Fibrillary Acidic Protein / metabolism
  • Glutamic Acid / pharmacology
  • Homeodomain Proteins / metabolism
  • Immunohistochemistry / methods
  • Indoles
  • Intermediate Filament Proteins / metabolism
  • Membrane Potentials / drug effects
  • Membrane Potentials / radiation effects
  • Mice
  • Nerve Tissue Proteins / metabolism
  • Nestin
  • Neuroglia / metabolism
  • Neurogranin
  • Neurons / classification
  • Neurons / cytology*
  • Neurons / drug effects
  • Neurons / metabolism
  • PAX6 Transcription Factor
  • Paired Box Transcription Factors
  • Patch-Clamp Techniques
  • Quinoxalines / pharmacology
  • Receptor, Nerve Growth Factor
  • Receptor, trkA / metabolism
  • Receptors, AMPA / metabolism
  • Receptors, Nerve Growth Factor / metabolism
  • Repressor Proteins
  • Stem Cells / drug effects
  • Stem Cells / physiology*
  • Stem Cells / radiation effects
  • Synaptophysin
  • Tetrodotoxin / pharmacology
  • Time Factors
  • Tretinoin / pharmacology
  • Tubulin / metabolism
  • Valine / analogs & derivatives*
  • Valine / pharmacology

Substances

  • Calmodulin-Binding Proteins
  • Carrier Proteins
  • Excitatory Amino Acid Agonists
  • Excitatory Amino Acid Antagonists
  • Eye Proteins
  • Fabp7 protein, mouse
  • Fatty Acid-Binding Protein 7
  • Fatty Acid-Binding Proteins
  • GABA Antagonists
  • Glial Fibrillary Acidic Protein
  • Homeodomain Proteins
  • Indoles
  • Intermediate Filament Proteins
  • Nerve Tissue Proteins
  • Nes protein, mouse
  • Nestin
  • Nrgn protein, mouse
  • PAX6 Transcription Factor
  • Paired Box Transcription Factors
  • Pax6 protein, mouse
  • Quinoxalines
  • Receptor, Nerve Growth Factor
  • Receptors, AMPA
  • Receptors, Nerve Growth Factor
  • Repressor Proteins
  • Synaptophysin
  • Tubulin
  • beta3 tubulin, mouse
  • 2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline
  • Neurogranin
  • Glutamic Acid
  • Tetrodotoxin
  • DAPI
  • Tretinoin
  • 2-amino-5-phosphopentanoic acid
  • Receptor, trkA
  • Valine
  • glutamate receptor ionotropic, AMPA 1
  • Bicuculline