Immunity
Volume 47, Issue 4, 17 October 2017, Pages 697-709.e3
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Article
By Capturing Inflammatory Lipids Released from Dying Cells, the Receptor CD14 Induces Inflammasome-Dependent Phagocyte Hyperactivation

https://doi.org/10.1016/j.immuni.2017.09.010Get rights and content
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Highlights

  • CD14 endocytosis upon oxPAPC binding prevents inflammation via TLR4

  • CD14 endocytosis upon oxPAPC binding promotes inflammation via caspase-1 and -11

  • oxPAPC and its components induce IL-1 release from living (hyperactive) phagocytes

  • Conditions of cell hyperactivation induce non-lethal inflammatory sepsis in mice

Summary

A heterogeneous mixture of lipids called oxPAPC, derived from dying cells, can hyperactivate dendritic cells (DCs) but not macrophages. Hyperactive DCs are defined by their ability to release interleukin-1 (IL-1) while maintaining cell viability, endowing these cells with potent aptitude to stimulate adaptive immunity. Herein, we found that the bacterial lipopolysaccharide receptor CD14 captured extracellular oxPAPC and delivered these lipids into the cell to promote inflammasome-dependent DC hyperactivation. Notably, we identified two specific components within the oxPAPC mixture that hyperactivated macrophages, allowing these cells to release IL-1 for several days, by a CD14-dependent process. In murine models of sepsis, conditions that promoted cell hyperactivation resulted in inflammation but not lethality. Thus, multiple phagocytes are capable of hyperactivation in response to oxPAPC, with CD14 acting as the earliest regulator in this process, serving to capture and transport these lipids to promote inflammatory cell fate decisions.

Keywords

Toll-like Receptor
inflammasome
hyperactivation
interleukin-1
dendritic cells
macrophages
innate immunity
oxPAPC
SMOC

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