Current Biology
Volume 24, Issue 16, 18 August 2014, Pages 1854-1865
Journal home page for Current Biology

Article
PINK1 Triggers Autocatalytic Activation of Parkin to Specify Cell Fate Decisions

https://doi.org/10.1016/j.cub.2014.07.014Get rights and content
Under an Elsevier user license
open archive

Highlights

  • PINK1-Parkin operates as a damage-gated molecular switch for cell fate decisions

  • Parkin catalyzes ubiquitination of selective substrates in response to specific stimuli

  • Parkin is the E3 ligase for Mcl-1 and mediates valinomycin-induced apoptosis

  • PINK1 triggers autocatalytic activation of Parkin by phosphorylating Ser65 of Parkin

Summary

Background

The PINK1-Parkin pathway is known to play important roles in regulating mitochondria dynamics, motility, and quality control. Activation of this pathway can be triggered by a variety of cellular stress signals that cause mitochondrial damage. How this pathway senses different levels of mitochondrial damage and mediates cell fate decisions accordingly is incompletely understood.

Results

Here, we present evidence that PINK1-Parkin has both cytoprotective and proapoptotic functions. PINK1-Parkin operates as a molecular switch to dictate cell fate decisions in response to different cellular stressors. Cells exposed to severe and irreparable mitochondrial damage agents such as valinomycin can undergo PINK1-Parkin-dependent apoptosis. The proapoptotic response elicited by valinomycin is associated with the degradation of Mcl-1. PINK1 directly phosphorylates Parkin at Ser65 of its Ubl domain and triggers activation of its E3 ligase activity through an autocatalytic mechanism that amplifies its E3 ligase activity toward Mcl-1.

Conclusions

Autocatalytic activation of Parkin bolsters its accumulation on mitochondria and apoptotic response to valinomycin. Our results suggest that PINK1-Parkin constitutes a damage-gated molecular switch that governs cellular-context-specific cell fate decisions in response to variable stress stimuli.

Cited by (0)