Neuron
Volume 29, Issue 2, February 2001, Pages 385-399
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Evx1 Is a Postmitotic Determinant of V0 Interneuron Identity in the Spinal Cord

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Abstract

Interneurons in the ventral spinal cord are essential for coordinated locomotion in vertebrates. During embryogenesis, the V0 and V1 classes of ventral interneurons are defined by expression of the homeodomain transcription factors Evx1/2 and En1, respectively. In this study, we show that Evx1 V0 interneurons are locally projecting intersegmental commissural neurons. In Evx1 mutant embryos, the majority of V0 interneurons fail to extend commissural axons. Instead, they adopt an En1-like ipsilateral axonal projection and ectopically express En1, indicating that V0 interneurons are transfated to a V1 identity. Conversely, misexpression of Evx1 represses En1, suggesting that Evx1 may suppress the V1 interneuron differentiation program. Our findings demonstrate that Evx1 is a postmitotic determinant of V0 interneuron identity and reveal a critical postmitotic phase for neuronal determination in the developing spinal cord.

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4

These authors contributed equally to this work.

5

Present address: National Institute for Physiological Sciences, Okasaki, Aichi 444-8585, Japan.

6

Present address: Evotec Neurosciences, D-22525 Hamburg, Germany.

7

Present address: Incyte Pharmaceuticals, Palo Alto, California 94304.