[19] Intra-RNA and RNA—protein cross-linking techniques in Escherichia coli ribosomes

doi:10.1016/S0076-6879(88)64050-X

Methods in Enzymology

Volume 164, 1988, Pages 287-309

https://doi.org/10.1016/S0076-6879(88)64050-X Get rights and content

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This chapter describes the methods currently in use in the laboratory for the analysis of RNA-protein and intra-RNA cross-link sites in E. coli ribosomal RNA. The methods are still by no means ideal, and are continually being modified and improved. In particular, the resolution of the RNA-protein cross-linked complexes on two-dimensional gels is not yet as sharp as that of the corresponding intra-RNA cross-linked complexes. Nonetheless the system has enabled us to isolate a considerable number of individual complexes, and from the example shown that eleven specific cross-link sites could be identified, involving nine of the 30S ribosomal proteins linked to RNA sequences comprising 30–150 nucleotides. The total data accumulated so far include nearly 40 intra-RNA cross-link sites identified to within two or three nucleotides in the E. coli 30S and 50S subunits, as well as a similar number of RNA-protein cross-link sites involving 19 different ribosomal proteins.

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[19] Intra-RNA and RNA—protein cross-linking techniques in Escherichia coli ribosomes

Publisher Summary

J. Mol. Biol.

J. Mol. Biol.

J. Mol. Biol.

J. Mol. Biol.

Anal. Biochem.

J. Mol. Biol.

Anal. Biochem.

J. Mol. Biol.

J. Mol. Biol.

Anal. Biochem.

Anal. Biochem.

Anal. Biochem.

J. Immunol. Methods

Nucleic Acids Res.

Nucleic Acids Res.