An electron microscope study of the megacaryocyte of the rat bone marrow: I. The development of the demarcation membrane system and the platelet surface coat

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The origin and development of the demarcation membrane system (DMS) were studied in rat megacaryocytes using various approaches. For morphological studies whole-body perfusion-fixation was used. Horse radish peroxidase and Thorotrast injected intravenously showed that these substances passed into the DMS under in vivo conditions at all stages of DMS development. Staining during fixation of megacaryocytes with ruthenium red or lanthanum ions showed that these substances passed into the cavities of the DMS and stained a material coating the membranes of the DMS. It was observed that DMS formation is initiated by tubular invaginations at multiple sites of the plasma membrane of the megacaryocyte, and it is suggested that further formation of DMS membranes may take place at localized foci in the DMS called “dense compartmentsrd. The dense compartments are in continuity with the DMS, but contain an electron dense substance and are coated on their cytoplasmic aspects with a granular material.

It is concluded that (a) the DMS is a derivative of the plasma membrane of the megacaryocyte, (b) the cavities of the DMS are continuous with and are part of the extracellular space at all stages of DMS development, and (c) the surface coat of rat platelets, which is stainable with ruthenium red and lanthanum ions, and which contains an acid mucopolysaccharide, is derived from the megacaryocyte.

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