Staufen protein associates with the 3′UTR of bicoid mRNA to form particles that move in a microtubule-dependent manner

doi:10.1016/0092-8674(94)90013-2

Cell

Volume 79, Issue 7, 30 December 1994, Pages 1221-1232

https://doi.org/10.1016/0092-8674(94)90013-2 Get rights and content

Abstract

Staufen protein is required in order to anchor bicoid (bcd) mRNA at the anterior pole of the Drosophila egg. Here we show that staufen protein colocalizes with bcd mRNA at the anterior, and that this localization depends upon its association with the mRNA. Upon injection into the embryo, bcd transcripts specifically interact with staufen, and we have mapped the sequences required to three regions of the 3′UTR, each of which is predicted to form a long stem-loop. The resulting staufen-bcd 3′UTR complexes form particles that show a microtubule-dependent localization. Since staufen is also transported with oskar (osk) mRNA during oogenesis, staufen associates specifically with both osk and bcd mRNAs to mediate their localizations, but at two distinct stages of development.

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Cell

ArticleStaufen protein associates with the 3′UTR of bicoid mRNA to form particles that move in a microtubule-dependent manner

Abstract

J. Mol. Biol.

Curr. Biol.

Cell

Cell

Cell

Cell

Curr. Opin. Cell Biol.

Cell

Dev. Biol.

Trends Neurosci.

Cell

Cell

Cell

Transport and localization of exogenous myelin basic protein mRNA microinjected into oligodendrocytes

J. Cell Biol.

Structure of genes for virus-associated RNAI and RNAII of adenovirus type 2

The role of localization of bicoid RNA in organizing the anterior pattern of the Drosophilla embryo

EMBO J.

Microtubule dynamics during the cell-cycle: the effect of taxol and nocodazole on the microtubule system of Pt K2 cells at different stages of the mitotic cycle

Int. Rev. Cytol.

Maternal control of anterior development

Autonomous determination of anterior structures in the early Drosophila embryo by the bicoid morphogen

Development

Cytoarchitecture and the patterning of fushi tarazu expression in the Drosophila blastoderm

Genes Dev.

Induction of germ cell formation by oskar

Nature

Maternal genes required for the anterior localization of bicoid activity in the embryo of Drosophila

Genes Dev.

Isolation and characterization of sex-linked female-sterile mutants in Drosophila melanogaster

Genetics

Localization of nanos RNA controls embryonic polarity

Cell

Article
Staufen protein associates with the 3′UTR of bicoid mRNA to form particles that move in a microtubule-dependent manner