Table 1.

Cells with monocyte surface markers in EV-containing tissue areas (skin).

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  • Using StrataQuest software, cells were identified and quantified in EV-containing areas through propidium iodide (PI) assessment (41.47% of all cells were found in EV area, first two images). Subsequently, MELC images for monocyte markers (CD11b+/Ly6C+/Ly6G) were superimposed and colocalizing signals were assessed (EV and markers: 28%). A similar approach was taken to assess neutrophils (CD45+/Ly6C+/Ly6G+) and monocytes in EVca and EVpa areas described in Fig 6A. Scale bars represent 100 μm.