RT Journal Article
SR Electronic
T1 Competition in the chaperone-client network subordinates cell-cycle entry to growth and stress
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e201800277
DO 10.26508/lsa.201800277
VO 2
IS 2
A1 David F Moreno
A1 Eva Parisi
A1 Galal Yahya
A1 Federico Vaggi
A1 Attila Csikász-Nagy
A1 Martí Aldea
YR 2019
UL https://www.life-science-alliance.org/content/2/2/e201800277.abstract
AB The precise coordination of growth and proliferation has a universal prevalence in cell homeostasis. As a prominent property, cell size is modulated by the coordination between these processes in bacterial, yeast, and mammalian cells, but the underlying molecular mechanisms are largely unknown. Here, we show that multifunctional chaperone systems play a concerted and limiting role in cell-cycle entry, specifically driving nuclear accumulation of the G1 Cdk–cyclin complex. Based on these findings, we establish and test a molecular competition model that recapitulates cell-cycle-entry dependence on growth rate. As key predictions at a single-cell level, we show that availability of the Ydj1 chaperone and nuclear accumulation of the G1 cyclin Cln3 are inversely dependent on growth rate and readily respond to changes in protein synthesis and stress conditions that alter protein folding requirements. Thus, chaperone workload would subordinate Start to the biosynthetic machinery and dynamically adjust proliferation to the growth potential of the cell.