RT Journal Article
SR Electronic
T1 Human DNA polymerase delta is a pentameric holoenzyme with a dimeric p12 subunit
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e201900323
DO 10.26508/lsa.201900323
VO 2
IS 2
A1 Prashant Khandagale
A1 Doureradjou Peroumal
A1 Kodavati Manohar
A1 Narottam Acharya
YR 2019
UL https://www.life-science-alliance.org/content/2/2/e201900323.abstract
AB Human DNA polymerase delta (Polδ), a holoenzyme consisting of p125, p50, p68, and p12 subunits, plays an essential role in DNA replication, repair, and recombination. Herein, using multiple physicochemical and cellular approaches, we found that the p12 protein forms a dimer in solution. In vitro reconstitution and pull down of cellular Polδ by tagged p12 substantiate the pentameric nature of this critical holoenzyme. Furthermore, a consensus proliferating nuclear antigen (PCNA) interaction protein motif at the extreme carboxyl-terminal tail and a homodimerization domain at the amino terminus of the p12 subunit were identified. Mutational analyses of these motifs in p12 suggest that dimerization facilitates p12 binding to the interdomain connecting loop of PCNA. In addition, we observed that oligomerization of the smallest subunit of Polδ is evolutionarily conserved as Cdm1 of Schizosaccharomyces pombe also dimerizes. Thus, we suggest that human Polδ is a pentameric complex with a dimeric p12 subunit, and discuss implications of p12 dimerization in enzyme architecture and PCNA interaction during DNA replication.