RT Journal Article
SR Electronic
T1 The small and large ribosomal subunits depend on each other for stability and accumulation
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e201800150
DO 10.26508/lsa.201800150
VO 2
IS 2
A1 Brian Gregory
A1 Nusrat Rahman
A1 Ananth Bommakanti
A1 Md Shamsuzzaman
A1 Mamata Thapa
A1 Alana Lescure
A1 Janice M Zengel
A1 Lasse Lindahl
YR 2019
UL https://www.life-science-alliance.org/content/2/2/e201800150.abstract
AB The 1:1 balance between the numbers of large and small ribosomal subunits can be disturbed by mutations that inhibit the assembly of only one of the subunits. Here, we have investigated if the cell can counteract an imbalance of the number of the two subunits. We show that abrogating 60S assembly blocks 40S subunit accumulation. In contrast, cessation of the 40S pathways does not prevent 60S accumulation, but does, however, lead to fragmentation of the 25S rRNA in 60S subunits and formation of a 55S ribosomal particle derived from the 60S. We also present evidence suggesting that these events occur post assembly and discuss the possibility that the turnover of subunits is due to vulnerability of free subunits not paired with the other subunit to form 80S ribosomes.