TY - JOUR T1 - Reciprocal regulation of STING and TCR signaling by mTORC1 for T-cell activation and function JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.201800282 VL - 2 IS - 1 SP - e201800282 AU - Takayuki Imanishi AU - Midori Unno AU - Wakana Kobayashi AU - Natsumi Yoneda AU - Satoshi Matsuda AU - Kazutaka Ikeda AU - Takayuki Hoshii AU - Atsushi Hirao AU - Kensuke Miyake AU - Glen N Barber AU - Makoto Arita AU - Ken J Ishii AU - Shizuo Akira AU - Takashi Saito Y1 - 2019/02/01 UR - https://www.life-science-alliance.org/content/2/1/e201800282.abstract N2 - Stimulator of interferon genes (STING) plays a key role in detecting cytosolic DNA and induces type I interferon (IFN-I) responses for host defense against pathogens. Although T cells highly express STING, its physiological role remains unknown. Here, we show that costimulation of T cells with the STING ligand cGAMP and TCR leads to IFN-I production and strongly inhibits T-cell growth. TCR-mediated mTORC1 activation and sustained activation of IRF3 are required for cGAMP-induced IFN-I production, and the mTORC1 activity is partially counteracted by cGAMP, thereby blocking proliferation. This mTORC1 inhibition in response to costimulation depends on IRF3 and IRF7. Effector T cells produce much higher IFN-I levels than innate cells in response to cGAMP. Finally, we demonstrated that STING stimulation in T cells is effective in inducing antitumor responses in vivo. Our studies demonstrate that the outputs of STING and TCR signaling pathways are mutually regulated through mTORC1 to modulate T-cell functions. ER -