@article {Imanishie201800282, author = {Takayuki Imanishi and Midori Unno and Wakana Kobayashi and Natsumi Yoneda and Satoshi Matsuda and Kazutaka Ikeda and Takayuki Hoshii and Atsushi Hirao and Kensuke Miyake and Glen N Barber and Makoto Arita and Ken J Ishii and Shizuo Akira and Takashi Saito}, title = {Reciprocal regulation of STING and TCR signaling by mTORC1 for T-cell activation and function}, volume = {2}, number = {1}, elocation-id = {e201800282}, year = {2019}, doi = {10.26508/lsa.201800282}, publisher = {Life Science Alliance}, abstract = {Stimulator of interferon genes (STING) plays a key role in detecting cytosolic DNA and induces type I interferon (IFN-I) responses for host defense against pathogens. Although T cells highly express STING, its physiological role remains unknown. Here, we show that costimulation of T cells with the STING ligand cGAMP and TCR leads to IFN-I production and strongly inhibits T-cell growth. TCR-mediated mTORC1 activation and sustained activation of IRF3 are required for cGAMP-induced IFN-I production, and the mTORC1 activity is partially counteracted by cGAMP, thereby blocking proliferation. This mTORC1 inhibition in response to costimulation depends on IRF3 and IRF7. Effector T cells produce much higher IFN-I levels than innate cells in response to cGAMP. Finally, we demonstrated that STING stimulation in T cells is effective in inducing antitumor responses in vivo. Our studies demonstrate that the outputs of STING and TCR signaling pathways are mutually regulated through mTORC1 to modulate T-cell functions.}, URL = {https://www.life-science-alliance.org/content/2/1/e201800282}, eprint = {https://www.life-science-alliance.org/content/2/1/e201800282.full.pdf}, journal = {Life Science Alliance} }