TY - JOUR T1 - <em>Viperin</em> controls chikungunya virus–specific pathogenic T cell IFNγ Th1 stimulation in mice JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.201900298 VL - 2 IS - 1 SP - e201900298 AU - Guillaume Carissimo AU - Teck-Hui Teo AU - Yi-Hao Chan AU - Cheryl Yi-Pin Lee AU - Bernett Lee AU - Anthony Torres-Ruesta AU - Jeslin JL Tan AU - Tze-Kwang Chua AU - Siew-Wai Fong AU - Fok-Moon Lum AU - Lisa FP Ng Y1 - 2019/02/01 UR - https://www.life-science-alliance.org/content/2/1/e201900298.abstract N2 - Chikungunya virus (CHIKV) has been a worldwide threat since its reemergence in La Reunion Island in 2004. Expression of the interferon-stimulated protein Viperin correlates with viral load burden in patients, and studies in mice have demonstrated its role to limit disease severity against CHIKV infection. Using Viperin−/− mice, we aimed to understand the contribution of Viperin to the T-cell immune response against CHIKV. CD4 T-cell depletion in Viperin−/− mice showed that increased late acute joint inflammation (5–8 d postinfection) was exclusively mediated by T cells. Specifically, CHIKV-infected Viperin−/− mice showed an increased INFγ Th1 profile of CD4 T cells, enhanced INFγ stimulation by APCs, an increased INFγ secretion profile in the joint microenvironment, and increased numbers of inflammatory monocytes in virus-infected joints compared with WT mice. Bone marrow grafting experiments showed that Viperin expression in both hematopoietic and non-hematopoietic cells is instrumental in reducing disease severity associated with a CD4 T-cell response. ER -