PT  - JOURNAL ARTICLE
AU  - Masashi Minamino
AU  - Torahiko L Higashi
AU  - Céline Bouchoux
AU  - Frank Uhlmann
TI  - Topological in vitro loading of the budding yeast cohesin ring onto DNA
AID  - 10.26508/lsa.201800143
DP  - 2018 Oct 01
TA  - Life Science Alliance
PG  - e201800143
VI  - 1
IP  - 5
4099  - https://www.life-science-alliance.org/content/1/5/e201800143.short
4100  - https://www.life-science-alliance.org/content/1/5/e201800143.full
SO  - Life Sci. Alliance2018 Oct 01; 1
AB  - The ring-shaped chromosomal cohesin complex holds sister chromatids together by topological embrace, a prerequisite for accurate chromosome segregation. Cohesin plays additional roles in genome organization, transcriptional regulation, and DNA repair. The cohesin ring includes an ABC family ATPase, but the molecular mechanism by which the ATPase contributes to cohesin function is not yet understood. In this study, we have purified budding yeast cohesin, as well as its Scc2–Scc4 cohesin loader complex, and biochemically reconstituted ATP-dependent topological cohesin loading onto DNA. Our results reproduce previous observations obtained using fission yeast cohesin, thereby establishing conserved aspects of cohesin behavior. Unexpectedly, we find that nonhydrolyzable ATP ground state mimetics ADP·BeF2, ADP·BeF3−, and ADP·AlFx, but not a hydrolysis transition state analog ADP·VO43−, support cohesin loading. The energy from nucleotide binding is sufficient to drive the DNA entry reaction into the cohesin ring. ATP hydrolysis, believed to be essential for in vivo cohesin loading, must serve a subsequent reaction step. These results provide molecular insights into cohesin function and open new experimental opportunities that the budding yeast model affords.