TY - JOUR T1 - Chronic platelet-derived growth factor receptor signaling exerts control over initiation of protein translation in glioma JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.201800029 VL - 1 IS - 3 SP - e201800029 AU - Shuang Zhou AU - Vicky A Appleman AU - Christopher M Rose AU - Hyun Jung Jun AU - Juechen Yang AU - Yue Zhou AU - Roderick T Bronson AU - Steve P Gygi AU - Al Charest Y1 - 2018/06/01 UR - https://www.life-science-alliance.org/content/1/3/e201800029.abstract N2 - Activation of the platelet-derived growth factor receptors (PDGFRs) gives rise to some of the most important signaling pathways that regulate mammalian cellular growth, survival, proliferation, and differentiation and their misregulation is common in a variety of diseases. Herein, we present a comprehensive and detailed map of PDGFR signaling pathways assembled from literature and integrate this map in a bioinformatics protocol designed to extract meaningful information from large-scale quantitative proteomics mass spectrometry data. We demonstrate the usefulness of this approach using a new genetically engineered mouse model of PDGFRα-driven glioma. We discovered that acute PDGFRα stimulation differs considerably from chronic receptor activation in the regulation of protein translation initiation. Transient stimulation activates several key components of the translation initiation machinery, whereas the clinically relevant chronic activity of PDGFRα is associated with a significant shutdown of translational members. Our work defines a step-by-step approach to extract biologically relevant insights from global unbiased phospho-protein datasets to uncover targets for therapeutic assessment. ER -