RT Journal Article SR Electronic T1 Chronic platelet-derived growth factor receptor signaling exerts control over initiation of protein translation in glioma JF Life Science Alliance JO Life Sci. Alliance FD Life Science Alliance LLC SP e201800029 DO 10.26508/lsa.201800029 VO 1 IS 3 A1 Shuang Zhou A1 Vicky A Appleman A1 Christopher M Rose A1 Hyun Jung Jun A1 Juechen Yang A1 Yue Zhou A1 Roderick T Bronson A1 Steve P Gygi A1 Al Charest YR 2018 UL https://www.life-science-alliance.org/content/1/3/e201800029.abstract AB Activation of the platelet-derived growth factor receptors (PDGFRs) gives rise to some of the most important signaling pathways that regulate mammalian cellular growth, survival, proliferation, and differentiation and their misregulation is common in a variety of diseases. Herein, we present a comprehensive and detailed map of PDGFR signaling pathways assembled from literature and integrate this map in a bioinformatics protocol designed to extract meaningful information from large-scale quantitative proteomics mass spectrometry data. We demonstrate the usefulness of this approach using a new genetically engineered mouse model of PDGFRα-driven glioma. We discovered that acute PDGFRα stimulation differs considerably from chronic receptor activation in the regulation of protein translation initiation. Transient stimulation activates several key components of the translation initiation machinery, whereas the clinically relevant chronic activity of PDGFRα is associated with a significant shutdown of translational members. Our work defines a step-by-step approach to extract biologically relevant insights from global unbiased phospho-protein datasets to uncover targets for therapeutic assessment.