RT Journal Article
SR Electronic
T1 CHRAC/ACF contribute to the repressive ground state of chromatin
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e201800024
DO 10.26508/lsa.201800024
VO 1
IS 1
A1 Alessandro Scacchetti
A1 Laura Brueckner
A1 Dhawal Jain
A1 Tamas Schauer
A1 Xu Zhang
A1 Frank Schnorrer
A1 Bas van Steensel
A1 Tobias Straub
A1 Peter B Becker
YR 2018
UL https://www.life-science-alliance.org/content/1/1/e201800024.abstract
AB The chromatin remodeling complexes chromatin accessibility complex and ATP-utilizing chromatin assembly and remodeling factor (ACF) combine the ATPase ISWI with the signature subunit ACF1. These enzymes catalyze well-studied nucleosome sliding reactions in vitro, but how their actions affect physiological gene expression remains unclear. Here, we explored the influence of Drosophila melanogaster chromatin accessibility complex/ACF on transcription by using complementary gain- and loss-of-function approaches. Targeting ACF1 to multiple reporter genes inserted at many different genomic locations revealed a context-dependent inactivation of poorly transcribed reporters in repressive chromatin. Accordingly, single-embryo transcriptome analysis of an Acf knock-out allele showed that only lowly expressed genes are derepressed in the absence of ACF1. Finally, the nucleosome arrays in Acf-deficient chromatin show loss of physiological regularity, particularly in transcriptionally inactive domains. Taken together, our results highlight that ACF1-containing remodeling factors contribute to the establishment of an inactive ground state of the genome through chromatin organization.