Development
- ETV2 induces endothelial, but not hematopoietic, lineage specification in birds
ETV2, a master regulator of blood and vessel development in mammals, is deleted in bird genomes, and exogenous ETV2 induces endothelial lineage specification in nascent chicken mesoderm.
- Genetic mutation of Cep76 results in male infertility due to abnormal sperm tail composition
This study explores the role of CEP76 in male fertility via its predicted role in establishing a functional transition zone that mediates protein entry into the sperm tail.
- Genetic reprogramming with stem cells regenerates glomerular epithelial podocytes in Alport syndrome
Podocytes are the rate-limiting glomerular cells for type IV collagen production, and horizontal gene transfer or cell fusion with stem cells regenerates the renal parenchyma in Alport syndrome.
- Generation of marmoset primordial germ cell–like cells under chemically defined conditions
Marmoset primordial germ cells (PGCs) express SOX17, AP2Ɣ, and BLIMP1 in vivo, and under specific feeder-free conditions, marmoset iPSCs can be induced into PGC-like cells in vitro.
- Tight regulation of a nuclear HAPSTR1-HUWE1 pathway essential for mammalian life
The recently discovered HAPSTR1 protein facilitates a nuclear quality control pathway essential for mammalian life.
- SHIP1 deficiency causes inflammation-dependent retardation in skeletal growth
SHIP1 plays a cell-intrinsic role in osteoclast function and development, yet skeletal growth retardation in SHIP1-deficient mice is dependent on lymphocyte-induced inflammation.
- The RNA-binding protein Msi2 regulates autophagy during myogenic differentiation
This study identifies the RNA-binding protein Musashi2 as a novel regulator of myogenesis. Msi2 regulates myoblast differentiation via controlling autophagic protein LC3 expression. Loss of Msi2 results in defective skeletal muscle function in vivo.
- IFT88 maintains sensory function by localising signalling proteins along Drosophila cilia
DmIFT88 is dispensable to preserve the auditory ciliary structure but is critical to maintain sensory cilia function through binding and localisation of the ciliary proteins Iav and DmGucy2d.
- Chromatin targeting of the RNF12/RLIM E3 ubiquitin ligase controls transcriptional responses
The E3 ubiquitin ligase RNF12/RLIM is mutated in intellectual disability and controls developmental gene expression. We show that chromatin targeting of RNF12 is required for substrate ubiquitylation and transcriptional responses, providing insight into mechanisms by which ubiquitylation regulates gene expression.
- CLN3 deficiency leads to neurological and metabolic perturbations during early development
Cln3 CRISPR mutant zebrafish larvae display BMP depletion and accumulation of glycerophosphodiesters and cholesteryl esters at very early developmental stages, recapitulating key features of human CLN3 disease.