Table of Contents
Methods
- A high-throughput two-cell assay for interrogating inhibitory signaling pathways in T cells
This work describes a high-throughput cellular assay specifically designed to study the understudied inhibitory signalling pathway in T cells.
Research Articles
- tRF-1:30-Gly-CCC-3 inhibits thyroid cancer via binding to PC and modulating metabolic reprogramming
This study reports a mechanism of tRNA fragments in PTC whereby tRF-30 controls PC abundance and subsequent TCA cycle anaplerosis to affect metabolic reprogramming and cancer progression.
- Human RAD52 stimulates the RAD51-mediated homology search
This study sheds new molecular light on the role of RAD52 in homologous recombination using direct electron microscopy visualization of its interdependent activity with RAD51 and BRCA2 onto resected double strand break extremity.
- Changes in searching behaviour of CSL transcription complexes in Notch active conditions
Single molecule live imaging of the Notch pathway transcription factor CSL reveals how its properties change in Notch active nuclei, acquiring exploratory behaviour.
- Loss-of-function cancer-linked mutations in the EIF4G2 non-canonical translation initiation factor
This work identifies missense mutations in the translation initiation factor EIF4G2 gene in cancer, showing loss-of-function effects on binding to interacting proteins and translational activity.
- Extreme trait GWAS (Et-GWAS): Unraveling rare variants in the 3,000 rice genome
Using extreme bulk pooling and allele frequency assessment, the graphical user interface (GUI)-enabled association technique successfully revealed relationships between genetic variations and grain yield under drought stress condition.
- VE-cadherin junction dynamics in initial lymphatic vessels promotes lymph node metastasis
Exposure of initial dermal lymphatics to VEGFA or a tumor results in zippering of VE-cadherin at junctions, concomitant with Src-dependent VE-cadherin fragmentation.
- Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model
Repeated blood stage infections with the human malaria parasite, Plasmodium vivax, induce sterile strain-specific and partial strain-transcendent protection in the Aotus monkey model.
- The memory of airway epithelium damage in smokers and COPD patients
This study demonstrates that the COPD bronchial epithelium retains a memory of past injuries, allowing for prolonged in vitro replication of its abnormal phenotype, showcasing altered differentiation, barrier dysfunction, and epithelial-to-mesenchymal transition, thereby suggesting potential epigenetic modifications in its progenitors.
- 2′,3′ cyclic nucleotide 3′ phosphodiesterase 1 functional isoform antagonizes HIV-1 particle assembly
CNP1, not CNP2, is the primary isoform inhibiting HIV-1, highlighting posttranslational regulation in CNP antiviral role.
- Pridopidine subtly ameliorates motor skills in a mouse model for vanishing white matter
Assessment of the therapeutic efficacy of 4-PBA, TUDCA, and pridopidine in a vanishing white matter mouse model shows that only pridopidine subtly ameliorates motor skills.
- Tmprss2 maintains epithelial barrier integrity and transepithelial sodium transport
The membrane-bound serine protease Tmprss2 is required for ENaC-mediated transepithelial sodium and EpCAM-mediated paracellular transport in kidney cells.
- Early Atf4 activity drives airway club and goblet cell differentiation
PERK/Atf4 drives airway epithelial differentiation, impacting secretory cell numbers, revealing ATF4’s role in respiratory tract regeneration.
- Variable PD-1 glycosylation modulates the activity of immune checkpoint inhibitors
Two anti-PD-1 antibodies bind to distinct glycoforms of PD-1 found on cells or in blood, highlighting the potential of target glycoprofiling in the development of differentiated therapeutics.
- Centriole and transition zone structures in photoreceptor cilia revealed by cryo-electron tomography
Cryo-electron tomography and subtomogram averaging reveal new structural features at the base of the light-sensing cilia of retinal rods, including the basal body, the Y-links between axoneme and membrane, and the ciliary necklace of the transition zone.
- USP27X variants underlying X-linked intellectual disability disrupt protein function via distinct mechanisms
This study uncovers mechanisms by which X-linked intellectual disability disorder 105 (#300984; OMIM) variants alter USP27X biology and function.
- CLN3 deficiency leads to neurological and metabolic perturbations during early development
Cln3 CRISPR mutant zebrafish larvae display BMP depletion and accumulation of glycerophosphodiesters and cholesteryl esters at very early developmental stages, recapitulating key features of human CLN3 disease.
- Chromatin targeting of the RNF12/RLIM E3 ubiquitin ligase controls transcriptional responses
The E3 ubiquitin ligase RNF12/RLIM is mutated in intellectual disability and controls developmental gene expression. We show that chromatin targeting of RNF12 is required for substrate ubiquitylation and transcriptional responses, providing insight into mechanisms by which ubiquitylation regulates gene expression.
- The origin, evolution, and molecular diversity of the chemokine system
Extensive phylogenetic analysis of chemokine signalling molecules reveals the origin and evolution of canonical and non-canonical components, shedding light on the evolution of this complex system.
- Enhancer mutations modulate the severity of chemotherapy-induced myelosuppression
Using transcriptome and interactome maps with patients’ WGS, this research uncovers the role of noncoding mutations in the myelosuppression predisposition, advancing personalized cancer treatment.
Resources
- Single-cell time series analysis reveals the dynamics of HSPC response to inflammation
Unbiased single-cell time series analysis of gene expression response to inflammation, using novel computational approaches, links myeloid depletion to alterations in differentiation bias and pyroptosis activity.
- Gene architecture is a determinant of the transcriptional response to bulky DNA damages
A comparative genomic study of the transcriptomic response to bulky DNA damages uncovers novel response genes and the features of their structure that allow them to be better repaired and transcribed.