Table of Contents
Research Articles
- JIP2 haploinsufficiency contributes to neurodevelopmental abnormalities in human pluripotent stem cell–derived neural progenitors and cortical neurons
Molecular and cellular profiling of patient-specific neural cell types provides suggestions for the involvement of JIP2 in the neurodevelopmental disorder Phelan–McDermid syndrome.
- Dynamic m6A methylation facilitates mRNA triaging to stress granules
m6A modification in the 5′ vicinity of the coding sequence of transcripts provides a selective mechanism for triaging mRNAs to stress granules and is mediated by the YTHDF3 “reader” protein.
- The histone chaperone FACT modulates nucleosome structure by tethering its components
The histone chaperone FACT functions by tethering partial components of the nucleosome, thereby assisting nucleosome disassembly and reassembly during transcription.
- Immunoediting is not a primary transformation event in a murine model of MLL-ENL AML
Using a conditional model of MLL-ENL–driven AML, Dudenhöffer-Pfeifer and Bryder show that NK cell and adaptive immunity influences little on leukemia initiation from normal cells. The data argue against immune escape as a universal primary transformation event in AML.
- Role of Cnot6l in maternal mRNA turnover
Mice lacking Cnot6l, a deadenylase component of the CCR4–NOT complex, are viable, but females have ∼40% smaller litters. Cnot6l is a maternal-effect gene acting in maternal mRNA degradation.
- Transformation-induced stress at telomeres is counteracted through changes in the telomeric proteome including SAMHD1
The authors apply telomeric chromatin analysis to identify factors that accumulate at telomeres during cellular transformation, promoting telomere replication and repair and counteracting oncogene-borne telomere replication stress.
- Proteins of generalist and specialist pathogens differ in their amino acid composition
Primitive amino acids and disorder content in secreted proteins are distinctively enriched in generalist compared with host-specific pathogens.
- Vnn1 pantetheinase limits the Warburg effect and sarcoma growth by rescuing mitochondrial activity
Expression of the Vnn1 pantetheinase by sarcomas is tumor suppressive by limiting the use of aerobic glycolysis for growth and rescuing mitochondrial activity through CoA regeneration.
- Pericytes promote skin regeneration by inducing epidermal cell polarity and planar cell divisions
This study reveals that mesenchymal stem cell–like dermal pericytes increase the number of epidermal stem and progenitor cells in human skin by promoting planar cell divisions within the proliferative compartment, without compromising skin differentiation—most likely via paracrine BMP-2 secretion.
- Distinctive features of lincRNA gene expression suggest widespread RNA-independent functions
Combining single-cell RNA-seq of mouse ESCs and NPCs, lincRNA gene deletions, conditional RNA depletion, and nuclear exosome profiling distinguishes RNA-dependent and RNA-independent lincRNA gene activities.
- RNase E cleavage shapes the transcriptome of Rhodobacter sphaeroides and strongly impacts phototrophic growth
This study identifies the cleavage sites of the endoribonuclease RNase E in the Rhodobacter sphaeroides transcriptome and demonstrates its effect on oxidative stress resistance and phototrophic growth.
- Beetle luciferases with naturally red- and blue-shifted emission
New crystal structures of red- and green blue–shifted beetle luciferases reveal that the color emission mechanism is dependent on the active site microenvironment affected by the conformation of loop regions.
- Neuron-specific inactivation of Wt1 alters locomotion in mice and changes interneuron composition in the spinal cord
This work demonstrates a role for the Wilms tumor protein Wt1 in the specification of neurons that are involved in the control of locomotion.
- Atomic force microscopy reveals structural variability amongst nuclear pore complexes
Structural heterogeneity is resolved in isolated nuclear envelopes, revealing the lamina network and nuclear pore complexes of different sizes, as well as different morphologies in their transport barrier and different binding of nuclear transport receptors in the barrier.
- Chromatin-mediated translational control is essential for neural cell fate specification
Chd5 loss links the up-regulation of ribosomal genes to enhanced translation, causing the untimely production of a master transcription factor that unleashes stem cells and alters cell fate.